Cytokeratin 4 Antibody

Images

 
Western Blot: Cytokeratin 4 Antibody [NBP1-33412] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 4 Antibody [NBP1-33412] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT4 protein stained by Cytokeratin 4 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody [NBP1-33412] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cytokeratin 4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 4. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 4 Antibody

  • CK4
  • CK-4
  • CYK4cytokeratin-4
  • Cytokeratin-4
  • FLJ31692
  • K4cytokeratin 4
  • keratin 4
  • keratin, type II cytoskeletal 4
  • Keratin-4
  • Type-II keratin Kb4

Background

Intermediate filaments are protein polymers which, together with actin filaments and microtubules, form the cytoskeleton of cells. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Expression of the genes encoding these stratification-related cytokeratins starts already in the basal cell layer and does not depend on vertical differentiation and detachment from the basal lamina. Results also show that simple epithelial and stratification-related cytokeratins can be coexpressed in basal cell layers of certain stratified epithelia such as esophagus (1). Mucous keratin CK4 is known to appear in epidermis under certain abnormal conditions. a dose-response study showed that the de novo appearance of K4 can be utilized as a sensitive test for retinoid bioactivity in epidermis in vivo (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT4