Cytokeratin 4 Antibody - BSA Free

Western Blot: Cytokeratin 4 Antibody [NBP1-33412] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody [NBP1-33412] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT4 protein stained by Cytokeratin 4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody [NBP1-33412] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.

Western Blot: Cytokeratin 4 Antibody [NBP1-33412] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 4 antibody (NBP1-33412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 4. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cytokeratin 4 Antibody - BSA Free

• CK4
• CK-4
• CYK4cytokeratin-4
• Cytokeratin-4
• FLJ31692
• K4cytokeratin 4
• keratin 4
• keratin, type II cytoskeletal 4
• Keratin-4
• Type-II keratin Kb4

Background

Intermediate filaments are protein polymers which, together with actin filaments and microtubules, form the cytoskeleton of cells. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Expression of the genes encoding these stratification-related cytokeratins starts already in the basal cell layer and does not depend on vertical differentiation and detachment from the basal lamina. Results also show that simple epithelial and stratification-related cytokeratins can be coexpressed in basal cell layers of certain stratified epithelia such as esophagus (1). Mucous keratin CK4 is known to appear in epidermis under certain abnormal conditions. a dose-response study showed that the de novo appearance of K4 can be utilized as a sensitive test for retinoid bioactivity in epidermis in vivo (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KRT4