Cytokeratin 20 Antibody (KRT20/7805R)

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 20 antibody (KRT20/7805R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Antibody (KRT20/7805R).

Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Antibody (KRT20/7805R).

• Reactivity: Hu
• Applications: IHC
• Clone: KRT20/7805R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 20 Antibody (KRT20/7805R) Summary

• Description: Positive Controls: HT29 cells. Human colon or prostate carcinoma.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Cytokeratin 20 protein (around aa aa324-424) (exact sequence is proprietary)
• Localization: Cytoplasm.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT20
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 20 Antibody (KRT20/7805R)

• CD20
• CK20
• CK-20
• cytokeratin-20
• K20cytokeratin 20
• keratin 20
• keratin, type I cytoskeletal 20
• keratin-20
• KRT21
• MGC35423
• Protein IT

Background

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT20