Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

Western Blot: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytoker

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 19.

Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of paraffin-embedded Kidney tissue using anti-Cytokeratin 19mouse monoclonal antibody.

Western Blot: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 19 monoclonal antibody.

Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-Cytokeratin 19 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Cytokeratin 19 mouse monoclonal antibody.

Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of paraffin-embedded liver tissue using anti-Cytokeratin 19mouse monoclonal antibody.

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free Summary

• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50 - 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 43.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

Cytokeratin 19 (also known as Keratin, type I cytoskeletal 19) belongs to the keratin family which consists of fibrous proteins that make up the framework of epithelial cells. Cytokeratin 19 plays a role in the organization of myofibers. This protein is known to have interactions with ZNF638, FANCG, FAM107A, KRT15 and EXOC8. Cytokeratin 19 has been studied in relation to several disorders and diseases including chordoma, adenoma, hamartoma, adenoiditis, carcinoma, and liver disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT19