Cytokeratin 19 Antibody (OTI3F8)

Western Blot: Cytokeratin 19 Antibody (3F8) [NBP1-48277] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 19 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 19.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of paraffin-embedded liver tissue using anti-Cytokeratin 19mouse monoclonal antibody.

Western Blot: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokeratin 19.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-Cytokeratin 19 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Cytokeratin 19 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of paraffin-embedded Kidney tissue using anti-Cytokeratin 19mouse monoclonal antibody.

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.15 mg/ml

Cytokeratin 19 Antibody (OTI3F8) Summary

• Description: Novus Biologicals Mouse Cytokeratin 19 Antibody (OTI3F8) (NBP1-48277) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
• Marker: Epithelial Cell Marker
• Specificity: This antibody is specific for Homo sapiens keratin 19 (KRT19).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT19
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 43.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.15 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytokeratin 19 Antibody (OTI3F8)

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Background

Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT19
• Entrez:
  • 3880(Human)
• Uniprot:
  • P08727()