Cytokeratin 19 Antibody (KRT19/1959R)

Western Blot: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693] - Western Blot Analysis of Hep-G2 cell lysate using Cytokeratin 19 Rabbit Recombinant Monoclonal Antibody (KRT19/1959R).

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693] - Formalin-fixed, paraffin-embedded human Colon stained with Cytokeratin 19 Rabbit Recombinant Monoclonal Antibody (KRT19/1959R).

• Reactivity: Hu, Mu
• Applications: WB, IHC-P
• Clone: KRT19/1959R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 19 Antibody (KRT19/1959R) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human full-length Cytokeratin 19 protein
• Localization: Cytoplasmic
• Specificity: This antibody reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa. It reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. This antibody is also a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT19
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08635)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 19 Antibody (KRT19/1959R)

• 40-kDa keratin intermediate filament
• CK19
• CK-19
• Cytokeratin 19
• EndoC
• K19
• K1CS
• keratin 19
• keratin, type I cytoskeletal 19
• keratin, type I, 40-kd
• keratin-19
• Krt19
• MGC15366

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT19