| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P |
| Clone | RCK106 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 405 |
| Immunogen | Clone RCK106 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with cytokeratins from the human bladder carcinoma cell line T24. |
| Marker | Epithelial Marker |
| Specificity | RCK106 reacts exclusively with cytokeratin 18 in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP1-97714AF350 | |
| Alexa Fluor 405 | NBP1-97714AF405 | |
| Alexa Fluor 488 | NBP1-97714AF488 | |
| Alexa Fluor 532 | NBP1-97714AF532 | |
| Alexa Fluor 594 | NBP1-97714AF594 | |
| Alexa Fluor 647 | NBP1-97714AF647 | |
| Alexa Fluor 700 | NBP1-97714AF700 | |
| Alexa Fluor 750 | NBP1-97714AF750 | |
| Allophycocyanin | NBP1-97714APC | |
| Biotin | NBP1-97714B | |
| DyLight 350 | NBP1-97714UV | |
| DyLight 405 | NBP1-97714V | |
| DyLight 488 | NBP1-97714G | |
| DyLight 550 | NBP1-97714R | |
| DyLight 594 | NBP1-97714DL594 | |
| DyLight 650 | NBP1-97714C | |
| DyLight 680 | NBP1-97714FR | |
| DyLight 755 | NBP1-97714IR | |
| FITC | NBP1-97714F | |
| HRP | NBP1-97714H | |
| Janelia Fluor 549 | NBP1-97714JF549 | |
| Janelia Fluor 646 | NBP1-97714JF646 | |
| PE | NBP1-97714PE | |
| PerCP | NBP1-97714PCP |
Diseases for Cytokeratin 18 Antibody (NBP1-97714V)Discover more about diseases related to Cytokeratin 18 Antibody (NBP1-97714V).
| Pathways for Cytokeratin 18 Antibody (NBP1-97714V)View related products by pathway.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT18 |
![Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Cytokeratin-8-Antibody-Western-Blot-NBP2-16094-img0006.jpg)
![Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/images2/Cytokeratin 8 Antibody-Western Blot-NBP2-16094-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Cytokeratin-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16094-img0007.jpg)
![Western Blot: HOXB2 Antibody [NBP2-83055] - Host: Rabbit. Target Name: HOXB2. Sample Type: 721_B. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/images2/HOXB2-Antibody-Western-Blot-NBP2-83055-img0005.jpg)
![Western Blot: HOXB2 Antibody [NBP2-83055] - WB Suggested Anti-HOXB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/images2/HOXB2-Antibody-Western-Blot-NBP2-83055-img0001.jpg)
![Western Blot: HOXB2 Antibody [NBP2-83055] - Host: Rabbit. Target Name: HOXB2. Sample Type: HepG2. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/images2/HOXB2-Antibody-Western-Blot-NBP2-83055-img0002.jpg)
![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg)
![Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/images2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vimentin Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0008.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0007.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/images2/Cytokeratin-14-Antibody-(LL002)-Immunocytochemistry-Immunofluorescence-NBP2-34270-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).](http://images.novusbio.com/images2/Cytokeratin 14 Antibody (LL002)-Immunohistochemistry-Paraffin-NBP2-34270-img0011.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/Cytokeratin-14-Antibody-(LL002)-Flow-Cytometry-NBP2-34270-img0009.jpg)
![Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).](http://images.novusbio.com/images2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Western-Blot-NBP2-47940-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/images2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47940-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/images2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47940-img0009.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0031.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0042.jpg)
![Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Simple-Western-NB100-56708-img0033.jpg)
![Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, Cytokeratin 5, CK6 and CK14. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128096) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Cytokeratin-5-Antibody-(EP1601Y)-Western-Blot-NB110-56916-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.](http://images.novusbio.com/images2/Cytokeratin-5-Antibody-(EP1601Y)-Immunocytochemistry-Immunofluorescence-NB110-56916-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.](http://images.novusbio.com/images2/Cytokeratin-5-Antibody-(EP1601Y)-Immunohistochemistry-Paraffin-NB110-56916-img0009.jpg)
