Cytokeratin 18 Antibody (DA-7)

Product Discontinued

Cytokeratin 18 Antibody (DA-7) Summary

• Immunogen: Tissue / cell preparation (Human). (Breast carcinoma cell line PMC-42).
• Marker: Epithelial Marker
• Specificity: Human Cytokeratin peptide 18 (45 kDa).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry-Paraffin Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Predicted molecular weight: 45 kDa. Western Blot Application note: Non-reducing conditions. SDS-PAGE (10% separating gel).
• Control:

  Cytokeratin 18 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human. Does not cross-react with Porcine, Bovine, Canine, Mouse, Rat, Hamster and Sheep.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 18 Antibody (DA-7)

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 18 Antibody (NB500-353)(1)

Publication using NB500-353

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: KRT18
• Entrez:
  • 3875(Human)