Cytokeratin 18 Antibody (2G3) - BSA Free

Western Blot: Cytokeratin 18 Antibody (2G3) [NBP1-74034] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cytokeratin 18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysateLane 3.: MCF7 cell lysate

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (2G3) [NBP1-74034] - ICC/IF analysis of KRT18 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (2G3) [NBP1-74034] - Analysis in colorectal cancer tissue.

Product Discontinued

Cytokeratin 18 Antibody (2G3) - BSA Free Summary

• Immunogen: Recombinant human Cytokeratin 18 (79-430aa) purified from E. coli
• Marker: Epithelial Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Cytokeratin 18 Antibody (2G3) - BSA Free

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• Cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers. Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18
• Uniprot:
  • NP_000215(Human)