Cytokeratin 18 Antibody (2G3)


Western Blot: Cytokeratin 18 Antibody (AT2G3) [NBP1-74034] - SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (AT2G3) [NBP1-74034] - Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody more
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (2G3) [NBP1-74034] - Analysis in colorectal cancer tissue.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
1 mg/ml

Cytokeratin 18 Antibody (2G3) Summary

Recombinant human KRT18 (79-430aa) purified from E. coli
Epithelial Marker
Anti-human KRT18 mAb, clone 2G3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT18 protein.
IgG2b Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Liquid. In Phosphate-Buffered Saline (pH 7.4)
0.1% Sodium Azide
1 mg/ml
Protein G purified


Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 1000. Recommended starting dilution is 1:500

Reactivity Notes


Alternate Names for Cytokeratin 18 Antibody (2G3)

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18


Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers. Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-74034AF405
Alexa Fluor (R) 488 Labeled NBP1-74034AF488
Alexa Fluor (R) 647 Labeled NBP1-74034AF647
Alexa Fluor (R) 700 Labeled NBP1-74034AF700
Allophycocyanin Labeled NBP1-74034APC
DyLight 350 Labeled NBP1-74034UV
DyLight 405 Labeled NBP1-74034V
DyLight 405LS Labeled NBP1-74034V3
DyLight 488 Labeled NBP1-74034G
DyLight 680 Labeled NBP1-74034FR
DyLight 755 Labeled NBP1-74034IR
PE Labeled NBP1-74034PE
PerCP Labeled NBP1-74034PCP

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol KRT18

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