Cytokeratin 18 Antibody (2F8)

Western Blot: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8.Lane 1: KRT18 transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of monoclonal antibody to KRT18 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.

Western Blot: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - KRT18 monoclonal antibody (M01), clone 2F8 Analysis of KRT18 expression in HeLa.

Immunoprecipitation: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Analysis of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KRT18 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Cytokeratin 18 Antibody (2F8) [H00003875-M01] - Detection limit for recombinant GST tagged KRT18 is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

Cytokeratin 18 Antibody (2F8) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: KRT18 (AAH00180, 1 a.a. ~ 430 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSFTTRSTFSTNYRSLGSVQAPSYGARSVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRPASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRY
• Marker: Epithelial Marker
• Specificity: KRT18 - keratin 18
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytokeratin 18 Antibody (2F8)

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18
• Entrez:
  • 3875(Human)
• OMIM:
  • 148(Human)
• Uniprot:
  • AAH00180(Human)