Cytokeratin 15 Antibody (KRT15/1699)

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/1699) [NBP2-53307] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 15 Monoclonal Antibody (KRT15/1699).

• Reactivity: Hu, Rt, Bv
• Applications: Flow, IHC
• Clone: KRT15/1699
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 15 Antibody (KRT15/1699) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54463)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human Cytokeratin 15 protein (Uniprot: P19012)
• Localization: Cytoplasmic
• Marker: Esophageal Squamous Cell Carcinoma Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT15
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 15 Antibody (KRT15/1699)

• CK15
• CK-15
• cytokeratin 15
• Cytokeratin-15
• K15keratin-15, basic
• K1CO
• keratin 15
• keratin, type I cytoskeletal 15
• Keratin-15
• keratin-15, beta
• KRTB
• type I cytoskeletal 15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT15
• Uniprot:
  • P19012(Human)