Cytokeratin 15 Antibody (15K01)

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (15K01) [NBP1-40062] - Analysis of anti-KRT15 / Cytokeratin 15 antibody with human skin, hair follicle at concentration 5 ug/ml.

Product Discontinued

Cytokeratin 15 Antibody (15K01) Summary

• Immunogen: Synthetic peptide corresponding to 17 residues from the C-terminal of human Cytokeratin 15.
• Specificity: Recognizes human keratin 15.
• Isotype: IgG2 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT15
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 0.2% BSA and 40% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cytokeratin 15 Antibody (15K01)

• CK15
• CK-15
• cytokeratin 15
• Cytokeratin-15
• K15keratin-15, basic
• K1CO
• keratin 15
• keratin, type I cytoskeletal 15
• Keratin-15
• keratin-15, beta
• KRTB
• type I cytoskeletal 15

Background

A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues (1). Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath (2). In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT15
• Entrez:
  • 3866(Human)
• Uniprot:
  • P19012(Human)