Cytokeratin 14 Antibody (SPM263)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (20X) stained with Cytokeratin 14 MAb (SPM263).
Flow (Intracellular): Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - An intracellular stain was performed on HeLa cells with Cytokeratin 14 Antibody (SPM263) NBP2-34403AF647 (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (10X) stained with Cytokeratin 14 MAb (SPM263).
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Analysis of the AF647 conjugate of Cytokeratin 14 antibody (clone SPM263). An intracellular stain was performed on Jurkat cells with Cytokeratin 14 ...read more
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Analysis of the AF647 conjugate of Cytokeratin 14 antibody (clone SPM263). An intracellular stain was performed on Raji cells with Cytokeratin 14 antibody ...read more
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Analysis of the AF647 conjugate of Cytokeratin 14 antibody (clone SPM263). An intracellular stain was performed on THP-1 cells with Cytokeratin 14 ...read more
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Analysis of Alexa Fluor (R) 647 conjugate of Cytokeratin 14 antibody (clone SPM263). An intracellular stain was performed on Jurkat cells with Cytokeratin ...read more
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - An intracellular stain was performed on Daudi cells with Cytokeratin 14 (SPM263) antibody NBP2-34403APC (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - An intracellular stain was performed on HeLa cells with Cytokeratin 14 (SPM263) antibody NBP2-34403APC (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM263
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Cytokeratin 14 Antibody (SPM263) Summary

Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14
Localization
Cytoplasmic
Marker
Squamous Cell Marker
Specificity
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
KRT14
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Immunoprecipitation 1-2ug/500ug
  • Flow (Intracellular)
Application Notes
ELISA (For coating, order NBP2-34403 (BSA free version of NBP2-34355))
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 14 Antibody (SPM263)

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT14
Uniprot