Cytokeratin 10 Antibody (VIK-10) - BSA Free

Immunohistochemistry: Cytokeratin 10 Antibody (VIK-10) [NB500-354] - Fig. 1. Immunohistochemistry staining of skin basaliom (paraffin-embedded sections) with anti-human Cytokeratin 10 (VIK-10).

• Reactivity: Hu
• Applications: ICC/IF, IHC, IP
• Clone: VIK-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Cytokeratin 10 Antibody (VIK-10) - BSA Free Summary

• Description: Novus Biologicals Mouse Cytokeratin 10 Antibody (VIK-10) - BSA Free (NB500-354) is a monoclonal antibody validated for use in IHC, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction.
• Specificity: The antibody VIK-10 reacts with Cytokeratin 10 (56.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
• Application Notes: Immunohistochemistry-Paraffin sections - Pretreatment: trypsin/ temperature 37C
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 10 Antibody (VIK-10) - BSA Free

• BCIE
• BIE
• CK10
• CK-10
• cytokeratin 10
• Cytokeratin-10
• EHK
• K10keratosis palmaris et plantaris
• Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
• keratin 10
• keratin, type I cytoskeletal 10
• keratin-10
• KPP

Background

Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT10
• Entrez:
  • 3858(Human)