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Cytokeratin 10 Antibody (1C3D9)

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Western Blot: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Analysis of paraffin-embedded esophageal cancer tissues using KRT10 mouse mAb with DAB staining.
Western Blot: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Analysis using KRT10 mAb against human KRT10 (AA: 345-454) recombinant protein. (Expected MW is 38.7 kDa)
Western Blot: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Analysis using KRT10 mouse mAb against A431 (1), COS7 (2), Jurkat (3), and HEK293 (4) cell lysate.
Immunohistochemistry: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Analysis of paraffin-embedded lung cancer tissues using KRT10 mouse mAb with DAB staining.
ELISA: Cytokeratin 10 Antibody (1C3D9) [NBP2-61737] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
1C3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Cytokeratin 10 Antibody (1C3D9) Summary

Immunogen
Purified recombinant fragment of human KRT10 (AA: 345-454 ) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT10
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
58.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 10 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Cytokeratin 10 Antibody (1C3D9)

  • BCIE
  • BIE
  • CK10
  • CK-10
  • cytokeratin 10
  • Cytokeratin-10
  • EHK
  • K10keratosis palmaris et plantaris
  • Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
  • keratin 10
  • keratin, type I cytoskeletal 10
  • keratin-10
  • KPP

Background

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-29429
Dual RNAscope ISH-IHC: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for Pan Cytokeratin mRNA (ACD RNAScope probe, catalog # 310221; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-29429) at 0.3ug/mL for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution of 1:500, B) pan CK at a dilution of 1:200, C) pan CK at a dilution of 1:400, D) pan CK at a dilution of 1:800. Image from verified customer review.
Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).
(18 Publications)
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, Flow-IC, Single-Cell Western
Cytokeratin 14
Antibody (LL002)
NBP2-34270
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Involucrin
Antibody (SY5)
NB100-2727
Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.
Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).
(1 Review)
(1 Publication)
Species: Hu, Po, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Cytokeratin 19
Antibody
NB100-687
Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Cytokeratin 16
Antibody (OTI2H8)
NBP2-45538
Western Blot: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 16.
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC, IHC-P
Cytokeratin 5
Antibody (2C2)
NBP2-22194
Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.
(1 Review)
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Filaggrin Antibody
NBP1-87528
Immunocytochemistry/Immunofluorescence: Filaggrin Antibody [NBP1-87528] - Staining of human cell line HaCaT shows localization to vesicles.
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human kidney shows low expression as expected.
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Cytokeratin 13
Antibody (SN71-09)
NBP2-67689
Western Blot: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Staining Cytokeratin 13 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Fi, Ze
Applications: WB, ICC/IF, IHC, IHC-P
Cytokeratin 8
Antibody
NBP2-16094
Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KO
Loricrin Antibody
NBP1-33610
Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610] - Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). Specimens were incubated overnight with the primary antibody (1:100 dilution). Blue staining corresponds to nuclei while loricrin is visible in green. IHC-P image submitted by a verified customer review.
(3 Reviews)
(2 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Cytokeratin 17
Antibody
NBP2-38536
Western Blot: Cytokeratin 17 Antibody [NBP2-38536] - Analysis in human cell lines HeLa and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - MYP3 rat urothelial cell line with CK17 (green) and DAPI (blue). Primary antibody at 1:200. Image from verified customer review.
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining in human urinary bladder and endometrium tissues using anti-KRT17 antibody. Corresponding KRT17 RNA-seq data are presented for the same tissues.
(1 Review)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PCNA Antibody
(PC10)
NB500-106
Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
(4 Reviews)
(30 Publications)
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Choline kinase
alpha Antibody
NBP1-85630
Western Blot: Choline kinase alpha Antibody [NBP1-85630] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-CHKA antibody. Corresponding CHKA RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
Choline Kinase
beta [Unconjugated]
8184-CK
Choline Kinase beta [Unconjugated]
Species: Hu
Cytokeratin 7
Antibody (OV-TL12/30) ...
NBP2-47940
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
(1 Review)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IF
Kv6.1 Antibody
NBP1-81572
Western Blot: Kv6.1 Antibody [NBP1-81572] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: Kv6.1 Antibody [NBP1-81572] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Kv6.1 Antibody [NBP1-81572] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Vimentin Antibody
NB300-223
Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.
Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.
(6 Reviews)
(39 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
HOXB2 Antibody
NBP2-83055
Western Blot: HOXB2 Antibody [NBP2-83055] - Host: Rabbit. Target Name: HOXB2. Sample Type: 721_B. Antibody Dilution: 1.0ug/ml
Western Blot: HOXB2 Antibody [NBP2-83055] - WB Suggested Anti-HOXB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate
Western Blot: HOXB2 Antibody [NBP2-83055] - Host: Rabbit. Target Name: HOXB2. Sample Type: HepG2. Antibody Dilution: 1.0ug/ml
Species: Hu
Applications: WB

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Control Lysate(s)

Cytokeratin 10 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT10
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