Cytokeratin 1 Antibody (10W8J6)

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded mouse skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 10W8J6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cytokeratin 1 Antibody (10W8J6) Summary

• Description: Novus Biologicals Rabbit Cytokeratin 1 Antibody (10W8J6) (NBP3-16883) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (P04264). GGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cytokeratin 1 Antibody (10W8J6)

• CK1
• CK-1
• cytokeratin 1
• Cytokeratin-1
• cytokeratin-1,67 kDa cytokeratin
• EHK
• EHK1
• epidermolytic hyperkeratosis 1
• EPPK
• Hair alpha protein
• K1
• keratin 1
• keratin, type II cytoskeletal 1
• Keratin-1
• KRT1A
• KRTA
• NEPPK
• Type-II keratin Kb1

Background

Belonging to the keratin family, Cytokeratin 1 plays an important role in cell binding. More specifically, this gene is expressed in the spinous and granular layers of the epidermis with keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. This gene also regulates the activity of kinases such as PKC and SRC by binding to ITGB1 and the receptor of GNB2L1. Other biological processes include complement activation, lectin pathway, epidermis development, fibrinolysis, regulation of angiogenesis and response to oxidative stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT1