Cytochrome P450 1A1 Antibody (6G5) - BSA Free

Western Blot: Cytochrome P450 1A1 Antibody (6G5) [NBP2-37526] - Analysis using CYP1A1 mAb against human CYP1A1 (AA: 203-461) recombinant protein. (Expected MW is 60 kDa)

Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody (6G5) [NBP2-37526] - Analysis of rectum cancer tissues using CYP1A1 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody (6G5) [NBP2-37526] - Analysis of ovarian cancer tissues using CYP1A1 mouse mAb with DAB staining.

ELISA: Cytochrome P450 1A1 Antibody (6G5) [NBP2-37526] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Simple Western: Cytochrome P450 1A1 Antibody (6G5) [NBP2-37526] - Human foreskin cell lysates. Protein concentration is 400 ug/mL. CYP1A1 antibody is diluted 1:20. Detection with chemiluminescence. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, IHC
• Clone: 6G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytochrome P450 1A1 Antibody (6G5) - BSA Free Summary

• Description: Novus Biologicals Mouse Cytochrome P450 1A1 Antibody (6G5) - BSA Free (NBP2-37526) is a monoclonal antibody validated for use in IHC, WB, ELISA and Simple Western. Anti-Cytochrome P450 1A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Cytochrome P450 1A1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP1A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Simple Western 1:20
  • Western Blot 1:500 - 1:2000
• Application Notes: Use in Simple Western reported by customer review.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human foreskin cell lysates, separated by Size, antibody dilution of 1:20
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Cytochrome P450 1A1 Antibody (6G5) - BSA Free

• AHH
• AHRR
• aryl hydrocarbon hydroxylase
• CP11
• CYP1
• CYP1A1
• CYPIA1
• cytochrome P1-450, dioxin-inducible
• Cytochrome P450 1A1
• Cytochrome P450 form 6
• cytochrome P450, family 1, subfamily A, polypeptide 1
• Cytochrome P450-C
• Cytochrome P450-P1
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• P1-450
• P450-C
• P450DX
• subfamily I (aromatic compound-inducible), polypeptide 1
• xenobiotic monooxygenase

Background

The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 1A1 Antibody (NBP2-37526)(1)

Publication using NBP2-37526

• Valkonen, S;Holopainen, M;Colas, RA;Impola, U;Dalli, J;Kakela, R;Siljander, PR;Laitinen, S; Lipid mediators in platelet concentrate and extracellular vesicles: Molecular mechanisms from membrane glycerophospholipids to bioactive molecules Biochim Biophys Acta Mol Cell Biol Lipids 2019-04-10 [PMID: 30980920] (WB, Human)

Review for Cytochrome P450 1A1 Antibody (NBP2-37526) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Irini Dijkhoff Simple Western Human 05/24/2019

Summary

• Application: Simple Western
• Sample Tested: Human foreskin, adult ski, engineered human skin, keratinocytes, HaCaT cells,reconstructed human epidermis
• Species: Human
• Lot: 160112

Comments

• Comments: JESS default run settings are used.

Bioinformatics

• Gene Symbol: CYP1A1