Cytochrome b245 alpha Overexpression Lysate (Native)

Images

 
Western Blot: Cytochrome b245 alpha Overexpression Lysate (Adult Normal) [NBL1-09657] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytochrome b245 alpha.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cytochrome b245 alpha Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200706 and based on accession number NM_000101. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cytochrome b-245, alpha polypeptide (CYBA), mRNA.
Gene
CYBA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cytochrome b245 alpha

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cytochrome b245 alpha Overexpression Lysate (Native)

  • cytochrome b light chain
  • Cytochrome b(558) alpha chain
  • cytochrome b(558) alpha-subunit
  • cytochrome b, alpha polypeptide
  • cytochrome b-245 light chain
  • cytochrome b-245, alpha polypeptide
  • Cytochrome b558 subunit alpha
  • flavocytochrome b-558 alpha polypeptide
  • Neutrophil cytochrome b 22 kDa polypeptide
  • p22 phagocyte B-cytochrome
  • p22phox
  • p22-PHOX
  • Superoxide-generating NADPH oxidase light chain subunit

Background

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-41291
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-790
Species: Hu, Mu, Po
Applications: WB, IHC-P, PEP-ELISA
NBP1-82542
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91823
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-33736
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84254
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-33463
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-500
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
Applications: WB, IHC, IHC-Fr, IHC-P
NB110-58849
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb, Sh
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31546
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-61666
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, ICC
NBP1-76919
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
MAB1904
Species: Hu
Applications: WB, IHC, ICC
H00011214-M10
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P

Publications for Cytochrome b245 alpha Lysate (NBL1-09657) (0)

There are no publications for Cytochrome b245 alpha Lysate (NBL1-09657).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cytochrome b245 alpha Lysate (NBL1-09657) (0)

There are no reviews for Cytochrome b245 alpha Lysate (NBL1-09657). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cytochrome b245 alpha Lysate (NBL1-09657) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Cytochrome b245 alpha Products

Bioinformatics Tool for Cytochrome b245 alpha Lysate (NBL1-09657)

Discover related pathways, diseases and genes to Cytochrome b245 alpha Lysate (NBL1-09657). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytochrome b245 alpha Lysate (NBL1-09657)

Discover more about diseases related to Cytochrome b245 alpha Lysate (NBL1-09657).
 

Pathways for Cytochrome b245 alpha Lysate (NBL1-09657)

View related products by pathway.

PTMs for Cytochrome b245 alpha Lysate (NBL1-09657)

Learn more about PTMs related to Cytochrome b245 alpha Lysate (NBL1-09657).

Blogs on Cytochrome b245 alpha

There are no specific blogs for Cytochrome b245 alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Nox4 Antibody
NB110-58849

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytochrome b245 alpha Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CYBA