Cystatin/Stefin Homolog Antibody (263423)

• Reactivity: Mu
• Applications: WB, IP
• Clone: 263423
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cystatin/Stefin Homolog Antibody (263423) Summary

• Immunogen: E. coli-derived recombinant mouse Cystatin/Stefin Homolog
  Ile2-Phe97
  Accession # P56567
• Specificity: Detects mouse Cystatin/Stefin Homolog in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatin D, F, S, SA, SN, recombinant mouse (rm) Cystatin A, B, C, E/M, rmFetuin A, rhFetuin B, rmHPRG, rmKininogen, or rhKininostatin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Csta1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin/Stefin Homolog Antibody (263423)

• AREI
• Cystatin H
• Cystatin/Stefin Homolog
• PSS4
• STF1
• STFA

Background

RIKEN cDNA clone 2010005H15 (Nucleotide Accession # AK008122) predicted an intracellular protein, which was similar to members of the cystatin superfamily (1‑4). The protein is designated here as mouse Cystatin/Stefin Homolog 2 (Cystatin H2) because the deduced amino acid sequence showed the highest identity (75% over the entire length) to that of mouse cystatin/stefin homolog (SwissProt Accession # P56567).

1. Carninci, P. and Hayashizaki, Y. (1999) Methods. Enzymol. 303:19.
2. Carninci, P. et al. (2000) Genome Res. 10:1617.
3. Shibata, K. et al. (2000) Genome Res. 10:1757.
4. Kawai, J. et al. (2001) Nature 409:685.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Csta1
• Uniprot:
  • P56567()