Cystatin C Antibody (Cyst24cc) - BSA Free

ELISA: Cystatin C Antibody (Cyst24) [NB100-62127] - Calibration curve One-step fluoroimmunoassay in streptavidin coated plates. Capture MAbs: Cyst24 and Cyst23 are biotinylated (200 ng/well). Detection MAbs: Cyst10, Cyst19, Cyst28 or Cyst13 are Eu3+- labelled (200 ng/ml). Incubation volume 100 ul.

Sandwich ELISA: Cystatin C Antibody (Cyst24) [NB100-62127] - Titration curve of pooled normal serum in Cyst24. Cyst10- Eu3plus sandwich fluoroimmunoassay.

• Reactivity: Hu
• Applications: ELISA
• Clone: Cyst24cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Cystatin C Antibody (Cyst24cc) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Cystatin C purified from human urine.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CST3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay
  • Sandwich ELISA
• Application Notes: Recommended pairs for sandwich immunoassay (capture-detection):
  
  Cyst24 - Cyst19, (NB100-62127/NBP1-05766)
  Cyst24 - Cyst28, (NB100-62127/NBP1-05765)
  Cyst23 - Cyst13, (NB100-62126/NBP1-05763)
• Control:

  Cystatin C Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cystatin C Antibody (Cyst24cc) - BSA Free

• ARMD11
• bA218C14.4 (cystatin C)
• CST3
• cystatin 3
• cystatin C (amyloid angiopathy and cerebral hemorrhage)
• Cystatin C
• cystatin-3
• cystatin-C
• Gamma-trace
• MGC117328
• Neuroendocrine basic polypeptide
• Post-gamma-globulin

Background

Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. The cystatin superfamily encompasses proteins that contain multiple cystatin like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. Cystatin C (Cystatin 3) , a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin C Antibody (NB100-62127)(1)

Publication using NB100-62127

• Thiriet PE, Medagoda D, Porro G, Guiducci C Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads Biosensors 2020-12-21 [PMID: 33371213]

Bioinformatics

• Gene Symbol: CST3
• Entrez:
  • 1471(Human)