Cystatin C Antibody (197821) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 197821
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

Cystatin C Antibody (197821) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cystatin C
  Ser27-Ala146
  Accession # P01034
• Specificity: Detects human Cystatin C in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human Cystatin A, B, D, E/M, F, S, SA, SN, X/Z/P, or recombinant mouse Cystatin C is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CST3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human Cystatin C Antibody (Catalog # MAB11962) ELISA Detection: Human Cystatin C Biotinylated Antibody (Catalog # BAM11961) Standard: Recombinant Human Cystatin C (Catalog # 1196-PI)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin C Antibody (197821) [Biotin]

• ARMD11
• bA218C14.4 (cystatin C)
• CST3
• cystatin 3
• cystatin C (amyloid angiopathy and cerebral hemorrhage)
• Cystatin C
• cystatin-3
• cystatin-C
• Gamma-trace
• MGC117328
• Neuroendocrine basic polypeptide
• Post-gamma-globulin

Background

Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

1. Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
2. Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
3. Abrahamson, M. (1994) Methods Enzymol. 244:685.
4. Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
5. Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cystatin C Antibody (BAM11961)(1)

Publication using BAM11961

• Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort. PLoS ONE, 2016-01-28;11(1):e0148073. 2016-01-28 [PMID: 26820481] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CST3
• Uniprot:
  • P01034()