CYP3A43 Antibody - BSA Free

Immunohistochemistry-Paraffin: CYP3A43 Antibody [NBP2-16064] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: CYP3A43 Antibody [NBP2-16064] - CYP3A43 antibody detects CYP3A43 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP3A43 antibody (NBP2-16064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: CYP3A43 Antibody [NBP2-16064] - CYP3A43 antibody detects CYP3A43 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CYP3A43 protein stained by CYP3A43 antibody (NBP2-16064) diluted at 1:200.Blue: Hoechst 33342 staining.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CYP3A43 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CYP3A43. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Microsome membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP3A43
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CYP3A43 Antibody - BSA Free

• cytochrome P450 3A43
• cytochrome P450 polypeptide 43
• cytochrome P450, family 3, subfamily A, polypeptide 43
• cytochrome P450, subfamily IIIA, polypeptide 43
• EC 1.14.14.1
• MGC119315
• MGC119316

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, it is unknown whether the enzyme is also involved in xenobiotic metabolism. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CYP3A43
• Uniprot:
  • Q9HB55(Human)