CYP39A1 Overexpression Lysate (Native)

Images

 
Western Blot: CYP39A1 Overexpression Lysate (Adult Normal) [NBP2-07001] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP39A1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CYP39A1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_057677. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CYP39A1, mRNA.
Gene
CYP39A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CYP39A1 Overexpression Lysate (Native)

  • Cytochrome P450 39A1
  • cytochrome P450, family 39, subfamily A, polypeptide 1
  • cytochrome P450, subfamily XXXIX (oxysterol 7 alpha-hydroxylase), polypeptide 1,24-hydroxycholesterol 7-alpha-hydroxylase
  • EC 1.14.13.99
  • hCYP39A1
  • Oxysterol 7-alpha-hydroxylase
  • oxysterol 7alpha-hydroxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-32433
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50217
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
MAB9120
Species: Hu
Applications: ICC
NB400-153
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-29939
Species: Hu
Applications: WB, Flow, IHC-P
NB400-157
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
NBP2-61816
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-33596
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-89680
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-24493
Species: Hu, Mu, Bv, Ch, Eq, Op, Pm
Applications: WB
PP-N4111-00
Species: Hu
Applications: WB, IHC, IP
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NB400-140
Species: Hu, Rb, Mu(-)
Applications: WB, ICC/IF, IHC, IHC-P
H00003000-M06
Species: Hu
Applications: WB, ELISA, ICC/IF
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
MAB7788
Species: Hu
Applications: WB, Simple Western
NBP1-54758
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-62664
Species: Hu
Applications: IHC, IHC-P

Publications for CYP39A1 Lysate (NBP2-07001) (0)

There are no publications for CYP39A1 Lysate (NBP2-07001).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CYP39A1 Lysate (NBP2-07001) (0)

There are no reviews for CYP39A1 Lysate (NBP2-07001). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CYP39A1 Lysate (NBP2-07001) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for CYP39A1 Lysate (NBP2-07001)

Discover related pathways, diseases and genes to CYP39A1 Lysate (NBP2-07001). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CYP39A1 Lysate (NBP2-07001)

Discover more about diseases related to CYP39A1 Lysate (NBP2-07001).
 

Pathways for CYP39A1 Lysate (NBP2-07001)

View related products by pathway.

PTMs for CYP39A1 Lysate (NBP2-07001)

Learn more about PTMs related to CYP39A1 Lysate (NBP2-07001).

Blogs on CYP39A1

There are no specific blogs for CYP39A1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CYP39A1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CYP39A1