CYP24A1 Antibody (HL1783) - Azide and BSA Free

Immunohistochemistry-Paraffin: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - CYP24A1 antibody [HL1783] detects CYP24A1 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. CYP24A1 stained by CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - Non-transfected (-) and transfected (+) A549 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/Immunofluorescence: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - CYP24A1 antibody [HL1783] detects CYP24A1 protein at mitochondria by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP24A1 stained by CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

Western Blot: CYP24A1 Antibody (HL1783) - Azide and BSA Free [NBP3-25427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (NBP3-25427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL1783
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CYP24A1 Antibody (HL1783) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CYP24A1 Antibody (HL1783) - Azide and BSA Free (NBP3-25427) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human CYP24A1
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CYP24A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CYP24A1 Antibody (HL1783) - Azide and BSA Free

• 24-OHase
• 25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• CP241
• CYP24
• CYP241,25-alphadihydroxyvitamin D3 24-hydroxylase
• CYP24A1
• Cytochrome P450 24A1
• cytochrome P450, family 24, subfamily A, polypeptide 1,24-OHase
• cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
• Cytochrome P450-CC24
• EC 1.14.13.n4
• EC 1.14.15.16
• exo-mitochondrial protein
• MGC126273
• MGC126274
• P450-CC24
• vitamin D 24-hydroxylase
• Vitamin D(3) 24-hydroxylase

Background

The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is involved in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP24A1