CYP1B1 Antibody (5E2) - BSA Free

Western Blot: CYP1B1 Antibody (5E2) [NBP2-50190] - Detection of CYP1B1 in tissue culture supernatant at a 1:10 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 5E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CYP1B1 Antibody (5E2) - BSA Free Summary

• Immunogen: Ovalbumin-conjugated synthetic peptide - PENFDPARFLDKDGL
• Specificity: Does not react with CYP1A1.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP1B1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: By IHC this antibody detected CYP1B1 in biopsy samples of cancers of the bladder, brain, breast, colon, connective tissue, esophagus, kidney, lung, lymph node, ovary, skin, stomach, testis and uterus, but not normal cells. Positive control(s): IHC: formalin-fixed, paraffin-embedded ovarian cancer sections; Western Blot: Recombinant human CYP1B1 expressed in lymphoblast microsomes.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CYP1B1 Antibody (5E2) - BSA Free

• aryl hydrocarbon hydroxylase
• CP1B
• CYP1B1
• CYPIB1
• cytochrome P450 1B1
• cytochrome P450, family 1, subfamily B, polypeptide 1
• cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3
• EC 1.14.14.1
• EC 4.2.1.152
• flavoprotein-linked monooxygenase
• GLC3A
• microsomal monooxygenase
• P4501B1
• primary infantile)
• xenobiotic monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CYP1B1 Antibody (NBP2-50190). (Showing 1 - 2 of 2 FAQ).

1. Can you share more details on your Western Blot validation of CYP1B1 Antibody (5E2) [NBP2-50190] ?
   • Our WB validation was performed on human recombinant CYP1B1 (rhCYP1B1).  The peptide immunogen used to raise the antibody [5E2) is conserved in mouse and rat, hence the antibody most likely will work well in those species.  
2. Do you suggest any tissue or cell line as a positive control for Western Blot of CYP1B1 Antibody (5E2) [NBP2-50190] ?
   • To the best of our knowledge, there are no cell lines (mouse or human) stably expressing CYP1B1.  In fact CYP1B1 expressing cell lines are in demand  to screen for CYP1B1 inhibitors/prodrugs. Some cell lines were reported to transcriptionally express CYP1B1 are SKOV3, A549 and SiHa. But the question remains whether the CYP1B1 protein present in these cells.  If you are planning to perform WB testing, the material which we recommend would be recombinant CYP1B1 or  CYP1B1 microsomes. 

Bioinformatics

• Gene Symbol: CYP1B1