Cyclin E1 Antibody

Images

 
Western Blot: Cyclin E1 Antibody [NBP2-16055] - Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Cyclin E Antibody [NBP2-16055] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cyclin E1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments ...read more
Western Blot: Cyclin E Antibody [NBP2-16055] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

Cyclin E1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Cyclin E1. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCNE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (85%), Rat (85%), Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyclin E1 Antibody

  • CCNE
  • CCNE1
  • CCNEcyclin Es
  • Cyclin E1
  • cyclin Et
  • G1/S-specific cyclin-E1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCNE1
Entrez
Uniprot