Cyclin D1 Antibody (328303)

Cyclin D1 was detected in immersion fixed COLO 205 human colorectal adenocarcinoma cell line using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB4314) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LPS suppressed EPCs cell viability via inducing cell pyroptosis. (a, b) NLRP3 was silenced in EPCs cells. (c) MTT assay was used to examine cell viability. (d) The expression status of Cyclin D1 and CDK2 were determined by Western blot analysis. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 328303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyclin D1 Antibody (328303) Summary

• Immunogen: E. coli-derived recombinant human Cyclin D1
  Met1-Ile295
  Accession # P24385
• Specificity: Detects human Cyclin D1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCND1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclin D1 Antibody (328303)

• B-cell lymphoma 1 protein
• BCL-1 oncogene
• Bcl-1
• BCL1D11S287E
• CCND1
• cyclin D1 (PRAD1: parathyroid adenomatosis 1)
• Cyclin D1
• Cyl-1
• G1/S-specific cyclin D1
• G1/S-specific cyclin-D1
• PRAD1 oncogene
• PRAD1
• PRAD1B-cell CLL/lymphoma 1
• U21B31

Background

Cyclin D1 is a 44 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin D1 Antibody (MAB4314)(5)

Publications using MAB4314

• Aranka Brockmueller, Constanze Buhrmann, Parviz Shayan, Mehdi Shakibaei Resveratrol induces apoptosis by modulating the reciprocal crosstalk between p53 and Sirt-1 in the CRC tumor microenvironment Frontiers in Immunology 2023-07-27 [PMID: 37575245]
• Aranka Brockmueller, Parviz Shayan, Mehdi Shakibaei Evidence That beta 1-Integrin Is Required for the Anti-Viability and Anti-Proliferative Effect of Resveratrol in CRC Cells International Journal of Molecular Sciences 2022-04-25 [PMID: 35563105]
• Yana Liu, Shihong Zhang, Zhiwei Xue, Xiaoxia Zhou, Lin Tong, Jiachen Liao et al. Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells Bioengineered 2021-12-20 [PMID: 34738867]
• Yanfang Gao, Hui Yang, Yanhong Wang, Jihua Tian, Rongshan Li, Xiaoshuang Zhou Evaluation of the inhibitory effect of tacrolimus combined with mycophenolate mofetil on mesangial cell proliferation based on the cell cycle International Journal of Molecular Medicine 2020-08-07 [PMID: 32945359]
• S Zeng, B Zhu, J Zeng, W Wu, C Jiang Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway Mol Med Rep, 2018-10-17;0(0):. 2018-10-17 [PMID: 30365080] (Western Blot, Human)

Review for Cyclin D1 Antibody (MAB4314) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Natalie Erdmann ICC Human 11/29/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: U266
• Species: Human
• Lot: XRM031510A

Comments

• Comments: Cells immersion-fixed in 4% paraformaldehyde 10 minutes room temperature; permeabilized 0.5% Triton X-100 5 minutes; blocked 10% donkey serum/PBS 1 hour at room temperature; MAB4314 1/30 overnight at 4oC; Alexa 647 donkey F(ab')2 anti-mouse IgG 1/1000 1 hour at room termperature; images acquired on Zeiss AxioImager Cy5 filter 500ms exposure.

Bioinformatics

• Gene Symbol: CCND1
• Uniprot:
  • P24385()