CYB5R3 Antibody

Images

 
Western Blot: CYB5R3 Antibody [H00001727-B01P] - Analysis of CYB5R3 expression in transfected 293T cell line by CYB5R3 polyclonal antibody. Lane 1: CYB5R3 transfected lysate(33.11 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CYB5R3 Antibody [H00001727-B01P] - Analysis of purified antibody to CYB5R3 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CYB5R3 Antibody Summary

Immunogen
CYB5R3 (NP_000389.1, 1 a.a. - 301 a.a.) full-length human protein. MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF
Specificity
CYB5R3 - cytochrome b5 reductase 3,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
CYB5R3
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CYB5R3 Antibody

  • B5RNADH-cytochrome b5 reductase 3
  • cytochrome b5 reductase 3
  • Cytochrome b5 reductase
  • DIA1NADH-cytochrome b5 reductase 3 membrane-bound form
  • diaphorase (NADH) (cytochrome b-5 reductase)
  • diaphorase-1
  • EC 1.6.2.2
  • NADH-cytochrome b5 reductase 3 soluble form

Background

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CYB5R3
Entrez
Uniprot