CYB5R3 Antibody

Western Blot: CYB5R3 Antibody [H00001727-B01P] - Analysis of CYB5R3 expression in transfected 293T cell line by CYB5R3 polyclonal antibody. Lane 1: CYB5R3 transfected lysate(33.11 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CYB5R3 Antibody [H00001727-B01P] - Analysis of purified antibody to CYB5R3 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

CYB5R3 Antibody Summary

• Immunogen: CYB5R3 (NP_000389.1, 1 a.a. - 301 a.a.) full-length human protein. MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF
• Specificity: CYB5R3 - cytochrome b5 reductase 3,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CYB5R3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CYB5R3 Antibody

• B5RNADH-cytochrome b5 reductase 3
• cytochrome b5 reductase 3
• Cytochrome b5 reductase
• DIA1NADH-cytochrome b5 reductase 3 membrane-bound form
• diaphorase (NADH) (cytochrome b-5 reductase)
• diaphorase-1
• EC 1.6.2.2
• NADH-cytochrome b5 reductase 3 soluble form

Background

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYB5R3
• Entrez:
  • 1727(Human)
• Uniprot:
  • NP_000389.1(Human)