CXXC1 Antibody

Western Blot: CXXC1 Antibody [NB100-56630] - analysis at 1:1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system (Pierce) and by exposing to the film for 15 minutes.

Product Discontinued

CXXC1 Antibody Summary

• Immunogen: This polyclonal antibody was raised against a mixture of synthetic peptides containing 73-90 and 641-656 amino acids residues of human CGBP.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXXC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Control:

  Human Spleen Whole Tissue Lysate (Adult Whole Normal)
  CXXC1 Overexpression Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CXXC1 Antibody

• CFP1CXXC-type zinc finger protein 1
• CGBPPHD finger and CXXC domain-containing protein 1
• CpG binding protein
• cpG-binding protein
• CXXC finger 1 (PHD domain)
• CXXC finger protein 1
• DNA-binding protein with PHD finger and CXXC domain
• hCGBP
• HsT2645
• PCCX12410002I16Rik
• PHF185830420C16Rik
• SPP1
• ZCGPC1
• zinc finger, CpG binding-type containing 1

Background

Human CpG binding protein (hCGBP) contains three cysteine-rich domains, two of which exhibit homology to the plant homeodomain finger domain and the third cysteine-rich domain conforms to the CXXC motif identified in DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein 1. A fragment of hCGBP that contains the CXXC domain binds to an oligonucleotide probe containing a single CpG site, and this complex is disrupted by distinct oligonucleotide competitors that also contain a CpG motif(s). CBGP plays an important part in embryonic viability and peri-implantation development. Native hCGBP is detected as an 88-kDa protein by Western analysis and is ubiquitously expressed. CGBP trans-activates promoters that contain CpG motifs but not promoters in which the CpG is ablated suggesting that hCGBP is a transcriptional activator that recognizes unmethylated CpG dinucleotides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXXC1 Antibody (NB100-56630)(1)

Publication using NB100-56630

• Ansari KI, Mishra BP, Mandal SS. Human CpG binding protein interacts with MLL1, MLL2 and hSet1 and regulates Hox gene expression. Biochim Biophys Acta. 2008-01-01 [PMID: 18082152] (WB, Human)
  
  Details:
  WB (human Flag-CGBP cell line), Fig. 2C.

Bioinformatics

• Gene Symbol: CXXC1
• Uniprot:
  • Q9P0U4(Human)