CXXC1 Overexpression Lysate (Native)


Western Blot: CXXC1 Overexpression Lysate (Adult Normal) [NBL1-09648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CXXC1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

CXXC1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC203937 and based on accession number NM_014593. The protein contains a C-terminal DDK tag.
Homo sapiens CXXC finger 1 (PHD domain) (CXXC1), transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
75.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for CXXC1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CXXC1 Overexpression Lysate (Native)

  • CFP1CXXC-type zinc finger protein 1
  • CGBPPHD finger and CXXC domain-containing protein 1
  • CpG binding protein
  • cpG-binding protein
  • CXXC finger 1 (PHD domain)
  • CXXC finger protein 1
  • DNA-binding protein with PHD finger and CXXC domain
  • hCGBP
  • HsT2645
  • PCCX12410002I16Rik
  • PHF185830420C16Rik
  • SPP1
  • ZCGPC1
  • zinc finger, CpG binding-type containing 1


Proteins that contain a CXXC motif within their DNA-binding domain, such as CXXC1, recognize CpG sequences and regulate gene expression (Carlone and Skalnik, 2001 [PubMed 11604496]).[supplied by OMIM]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the mid-coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CXXC1 Lysate (NBL1-09648) (0)

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Gene Symbol CXXC1