CXCR6 Antibody (221002) [Unconjugated]

BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 Monoclonal Antibody (Catalog # MAB2145) or (B) Rat IgG2B Isotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 221002
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR6 Antibody (221002) [Unconjugated] Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with mouse CXCR6
  Met1-Leu351
  Accession # Q9EQ16
• Specificity: Stains mouse CXCR6-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR6

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR6 Antibody (221002) [Unconjugated]

• Bonzo
• BONZOG-protein coupled receptor bonzo
• CD186 antigen
• CD186
• CDw186
• chemokine (C-X-C motif) receptor 6
• C-X-C chemokine receptor type 6
• CXCR6
• CXC-R6
• CXCR-6
• G protein-coupled receptor
• STRL33
• STRL33G-protein coupled receptor STRL33
• TYMSTRCD186

Background

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR6 Antibody (MAB2145)(3)

Publications using MAB2145

• T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice PLoS ONE, 2017;12(10):e0186997. 2017 [PMID: 29088306] (Flow Cytometry, Mouse)
• Xia Y, Yan J, Jin X, Entman M, Wang Y The chemokine receptor CXCR6 contributes to recruitment of bone marrow-derived fibroblast precursors in renal fibrosis. Kidney Int, 2014;86(2):327-37. 2014 [PMID: 24646857] (IHC, Mouse)
• Hattermann K, Ludwig A, Gieselmann V, Held-Feindt J, Mentlein R The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6. Mol. Cell. Neurosci., 2008;39(1):133-41. 2008 [PMID: 18619850] (ICC, Mouse)

Bioinformatics

• Gene Symbol: CXCR6
• Uniprot:
  • Q9EQ16()