CXCR2/IL-8RB Antibody (48311.211) - BSA Free

Flow Cytometry: CXCR2/IL-8 RB Antibody (48311.211) [NB100-1631] - Staining CXCR2 in Human PBMC by Flow cytometry. Cells were fixed in formaldehyde. Primary antibody used at a 1/100 dilution and incubated for 1 hour.

• Reactivity: Hu
• Applications: Flow, B/N
• Clone: 48311.211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CXCR2/IL-8RB Antibody (48311.211) - BSA Free Summary

• Immunogen: Human CXCR2 transfected NSO mouse myeloma cells.
• Localization: Integral membrane protein.
• Specificity: Monoclonal Anti-Human CXCR-2 reacts with CXCR-2 transfected cells and not with the parent cell line by flow cytometry. The antibody shows no cross-reactivity with human CXCR-1.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR2
• Purity: Protein G purified
• Endotoxin Note: Endotoxin level is <10 ng/mg antibody as determined by the LAL method.

Applications/Dilutions

• Dilutions:
  • Block/Neutralize 0.5 - 1.5 ul/ml
  • Flow Cytometry 1:10-1:1000
• Application Notes: The antibody will neutralize human cell surface CXCR-2 mediated-myloperoxidase release from human granulocytes induced by GROa. It will not block myeloperoxidase release induced by IL-8. Monoclonal Anti-Human CXCR-2 may be used to detect CXCR-2 present on human blood cells by flow cytometry. The antibody may be used to neutralize human cell surface CXCR-2 mediated bioactivity. For neutralization, a working concentration of 0.5-1.5 ug/ml of antibody will block 50% of the bioactivity due to 1 ug/ul recombinant human GROa in an assay measuring myeloperoxidase released from human granulocytes. For flow cytometry, the use of 10 ul at 25 ug/ml into a cell suspension containing 1-2.5 x 10^6 human blood cells is recommended. The volume of cell suspension can be variable. The volume of cell suspension must not exceed 200 ul.
• Control:

  CXCR2/IL-8RB Overexpression Lysate

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.2 mm-filtered solution in phosphate buffered saline containing carbohydrates.
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for CXCR2/IL-8RB Antibody (48311.211) - BSA Free

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The a- and b- families, with four cysteine residues, are the largest and best characterized. In the a-family, one amino acid separates the first two cysteine residues (CXC); in the b-family the two cysteine residues (CC) are adjacent to each other. The a-chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL-8), while those that do not, act on lymphocytes (such as IP-10 and MIG). Examples of chemokines under the b-family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the g-family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the *-family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells.1 Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR-1 (IL-8RA, or type I IL-8 receptor) and CXCR-2 (IL-8RB, or type II IL-8 receptor) have been shown to share approximately 77% amino acid sequence identity. IL-8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca2+ levels. Whereas CXCR-1 is highly specific for IL-8, CXCR-2 has broad specificity and has been shown to bind with high-affinity to other ELR motif containing a chemokines including GRO", GRO$, GRO(, NAP-2 and ENA-78. In contrast, PF4 and IP-10 (two chemokines that lack the ELR motif) have been shown to lack binding affinity for CXCR-2. CXCR-1 and CXCR-2 are expressed by neutrophils but not B lymphocytes or T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCR2
• Entrez:
  • 3579(Human)