CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

Fluorophore-linked immunosorbent assay: CXCR1/IL-8 RA Antibody (8F1-1-4) [NBP1-43339] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD181 (CXCR1) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE.

• Reactivity: Hu, Ha
• Applications: FLISA, Flow, IHC, IHC-P
• Clone: 8F1-1-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD181.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

• CD128
• CD181 antigen
• CD181
• CDw128aC-C
• chemokine (C-X-C motif) receptor 1
• CKR-1
• CMKAR1
• C-X-C chemokine receptor type 1
• CXCR1
• CXC-R1
• CXCR-1
• High affinity interleukin-8 receptor A
• IL-8 RA
• IL-8 receptor type 1
• IL-8R A
• IL8R1
• IL8RA
• IL8RAC-C-CKR-1
• IL8RBA
• interleukin 8 receptor, alpha
• interleukin-8 receptor type 1
• interleukin-8 receptor type A

Background

The 8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of its ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCR1
• Entrez:
  • 3577(Human)
• Uniprot:
  • P25024(Human)