Canine CXCL8/IL-8 ELISA Kit (Colorimetric)

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ELISA: Canine CXCL8/IL-8 ELISA Kit (Colorimetric) [NBP2-59967] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma, cell culture supernates, tissue homogenates
Standard Curve Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml

Order Details

Canine CXCL8/IL-8 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of dog IL-8. No significant cross-reactivity or interference between dog IL-8 and analogues was observed.
Standard Curve Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CXCL8

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Stop Solution
  2. Standard (Freeze dried)
  3. Sample Diluent
  4. Wash Buffer (25 x concentrate)
  5. TMB Substrate
  6. Assay plate (12 x 8 coated Microwells)
  7. Biotin-antibody Diluent
  8. Biotin-antibody (100 x concentrate)
  9. Adhesive Strip (For 96 wells)
  10. HRP-avidin Diluent
  11. Instruction manual
  12. HRP-avidin (100 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Canine CXCL8/IL-8 ELISA Kit (Colorimetric)

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CXCL8