CXCL8/IL-8 Antibody (105105) [Unconjugated]

Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant PorcineIL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL.

• Reactivity: Po
• Applications: WB, B/N, ELISA(Cap)
• Clone: 105105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL8/IL-8 Antibody (105105) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant porcine IL-8/CXCL8
  Ala26-Gln104
  Accession # CAA43461
• Specificity: Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, rhCXCL13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, rmCXCL13, recombinant rat (rr) CXCL1, rrCXCL3/CINC2 alpha, or rr3/CINC-2 beta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL8
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 1-6 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Porcine CXCL8/IL-8 Biotinylated Antibody (Catalog number BAF535)
  Standard: Recombinant Porcine CXCL8/IL-8 (Catalog number 535-IN)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL8/IL-8 Antibody (105105) [Unconjugated]

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro.

1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL8/IL-8 Antibody (MAB5351)(4)

Publications using MAB5351

• S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017;85(4):. 2017 [PMID: 28167667] (ELISA Development (Capture), Porcine)
• Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. Intensive Care Med, 2007;33(6):1085-93. 2007 [PMID: 17457574] (ELISA Development, Human)
• Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006;69(5):391-5. 2006 [PMID: 16604540] (Luminex Development, Porcine)
• Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model. Anesthesiology, 2004;100(4):861-70. 2004 [PMID: 15087621] (ELISA Development, Porcine)

Bioinformatics

• Gene Symbol: IL8
• Uniprot:
  • CAA43461()