CXCL7/NAP-2 Antibody (3B9) - Azide and BSA Free

Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.Lane 1: PPBP transfected lysate(13.9 KDa).Lane 2: Non-transfected lysate.

Western Blot: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi ( Cat # H00005473-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 (Cat # H00005473-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: CXCL7/NAP-2 Antibody (3B9) [H00005473-M01] - Detection limit for recombinant GST tagged PPBP is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, KD
• Clone: 3B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CXCL7/NAP-2 Antibody (3B9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPBP (AAH28217, 37 a.a. ~ 128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TKGQTKRNLAKGKEESLDSDLYAELRCMCIKTTSGIHPKNIQSLEVIGKGTHCNQVEVIATLKDGRKICLDPDAPRIKKIVQKKLAGDESAD
• Specificity: PPBP - pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPBP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA. IHC-Fr usage reported in scientific literature (PMID: 25858640).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CXCL7/NAP-2 Antibody (3B9) - Azide and BSA Free

• Beta-TG
• connective tissue-activating peptide III
• CTAP III
• CTAP3CTAP-III
• CTAPIII
• CXC chemokine ligand 7
• C-X-C motif chemokine 7
• CXCL7
• CXCL7b-TG1
• LA-PF4
• LDGFTC1
• Leukocyte-derived growth factor
• low-affinity platelet factor IV
• Macrophage-derived growth factor
• MDGFTC2
• NAP2
• NAP-2
• NAP-2-L1
• neutrophil-activating peptide 2
• neutrophil-activating peptide-2
• PBPTHBGB
• platelet basic protein
• PPBP
• pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• SCAR10
• SCYB7beta-thromboglobulin
• small inducible cytokine B7
• small inducible cytokine subfamily B, member 7
• Small-inducible cytokine B7
• TGB
• TGB1B-TG1
• THBGB1
• thrombocidin 1
• thrombocidin 2
• thromboglobulin, beta-1

Background

The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL7/NAP-2 Antibody (H00005473-M01)(1)

Publication using H00005473-M01

• Yeo L, Adlard N, Biehl M et al. Expression of chemokines CXCL4 and CXCL7 by synovial macrophages defines an early stage of rheumatoid arthritis Ann. Rheum. Dis. 2015-04-09 [PMID: 25858640] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: PPBP
• Entrez:
  • 5473(Human)
• OMIM:
  • 121010(Human)
• Uniprot:
  • AAH28217(Human)