CXCL6/GCP-2 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL6/GCP-2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CXCL6/GCP-2 (R&D Systems, Catalog # 333-GC)
  Val40-Asn114
  Accession # P80162
• Specificity: Detects human CXCL6/GCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP‑2, rhGRO alpha , rhGRO beta , rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP‑2, rhIL‑8, rhIP‑10, rhMIG, and rhSDF‑1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CXCL6/GCP-2 Antibody (Catalog # MAB333)
  ELISA Detection: Human CXCL6/GCP-2 Biotinylated Antibody (Catalog # BAF333)
  Standard: Recombinant Human CXCL6/GCP-2 (Catalog # 333-GC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL6/GCP-2 Antibody [Biotin]

• chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)
• Chemokine alpha 3
• CKA-3Granulocyte chemotactic protein 2
• CXCL6
• GCP-2
• GCP2member 6 (granulocytechemotactic protein 2)
• GCP-2Small-inducible cytokine B6
• Small inducible cytokine subfamily B (Cys-X-Cys), member b

Background

GCP-2 (granulocyte chemotactic protein-2) also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.

Human GCP-2 cDNA encodes a propeptide of 114 amino acid residues with a predicted 37 aa residue signal peptide and 77 aa residue mature protein. Several forms of natural GCP‑2 have been isolated from MG-63 conditioned media, indicating that GCP-2 undergoes limited processing at both the N- and C-termini. Human GCP-2 is a primary response gene whose induction by cytokines is attenuated by dexamethasone.

Human GCP-2 and mouse GCP-2/LIX have been shown to chemoattract and activate neutrophils, but not eosinophils and monocytes. It is likely that GCP-2 activities are mediated via the human or mouse CXCR2.

1. Proost, P. et al. (1993) J. Immunol. 150:1000.
2. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
3. Rovai, L.E. et al. (1997) J. Immunol. 158:5257.
4. Wuyts, A. et al. (1997) J. Immunol. 157:1736.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCL6
• Uniprot:
  • P80162()