Mouse CXCL4/PF4 ELISA Kit (Colorimetric)

ELISA: Mouse CXCL4/PF4 ELISA Kit (Colorimetric) [NBP2-59960] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse CXCL4/PF4 ELISA Kit (Colorimetric) [NBP2-59960] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 3.125 ng/mL - 200 ng/mL
• Sensitivity: 1.603 ng/mL

Mouse CXCL4/PF4 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PF-4. No significant cross-reactivity or interference between mouse PF-4 and analogues was observed.
• Standard Curve Range: 3.125 ng/mL - 200 ng/mL
• Sensitivity: 1.603 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PF4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CXCL4/PF4 ELISA Kit (Colorimetric)

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PF4