CXCL4/PF4 Antibody [Unconjugated]

Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL4/PF4 at approximately 15 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF795) followed by 1:50 dilution of HRP-conjugated Anti-Goat Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL4/PF4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL4/PF4
  Glu32-Ser101
  Accession # P02776
• Specificity: Detects human CXCL4/PF4 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse CXCL4/PF4 and recombinant rat CXCL4/PF4 is observed, and less than 2% cross-reactivity with recombinant human CXCL7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PF4

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL4/PF4 Antibody [Unconjugated]

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

1. Poncz, M. et al. (1987) Blood 69:219.
2. Scheuerer, B. et al. (2000) Blood 95:1158.
3. Perollet, C. et al. (1998) Blood 91:3289.
4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL4/PF4 Antibody (AF795)(1)

Publication using AF795

• van Bladel ER, Roest M Up-regulation of platelet activation in hemophilia A. Haematologica, 2011;0(0):. 2011 [PMID: 21422116] (ELISA Development, Human)

Reviews for CXCL4/PF4 Antibody (AF795) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous IP Mouse 01/09/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: See PMID 23009808
• Species: Mouse

Blocking

• Blocking Details: See PMID 23009808

Details

• Detection Notes: See PMID 23009808

Comments

• Comments: Published: See PMID 23009808Independent review from antibodyresource.com

reviewed by: Anonymous IP Human 01/09/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: See PMID 23009808
• Species: Human

Blocking

• Blocking Details: See PMID 23009808

Details

• Detection Notes: See PMID 23009808

Comments

• Comments: Published: See PMID 23009808Independent review from antibodyresource.com

Bioinformatics

• Gene Symbol: PF4
• Uniprot:
  • P02776()