CXCL4/PF4 Antibody [Unconjugated]

Western blot shows lysate of mouse platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL4/PF4 at approximately 14 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse CXCL4/PF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF595) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL4/PF4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL4/PF4
  Val30-Ser105
  Accession # Q9Z126
• Specificity: Detects mouse CXCL4/PF4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human CXCL4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PF4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL4/PF4 Antibody [Unconjugated]

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

1. Poncz, M. et al. (1987) Blood 69:219.
2. Scheuerer, B. et al. (2000) Blood 95:1158.
3. Perollet, C. et al. (1998) Blood 91:3289.
4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL4/PF4 Antibody (AF595)(3)

Publications using AF595

• Schroer, AB;Ventura, PB;Sucharov, J;Misra, R;Chui, MKK;Bieri, G;Horowitz, AM;Smith, LK;Encabo, K;Tenggara, I;Couthouis, J;Gross, JD;Chan, JM;Luke, A;Villeda, SA; Platelet factors attenuate inflammation and rescue cognition in ageing Nature 2023-08-16 [PMID: 37587343] (Western Blot, Transgenic Mouse, Mouse)
• Smeda M, Kieronska A, et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget 4/3/2018 [PMID: 29707148]
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)

Review for CXCL4/PF4 Antibody (AF595) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IP Mouse 01/08/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: See PMID 23009808
• Species: Mouse

Bioinformatics

• Gene Symbol: PF4
• Uniprot:
  • Q9Z126()