CXCL4/PF4 Antibody (170138) [Fluorescein]

Product Discontinued

CXCL4/PF4 Antibody (170138) [Fluorescein] Summary

• Immunogen: E. coli-derived recombinant human CXCL4/PF4
  Glu32-Ser101
  Accession # P02776.2
• Specificity: Detects human CXCL4/PF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) CXCL6, recombinant human (rh) CXCL6, rhCXCL7, rmCXCL13 or rhCCL21 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PF4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

1. Poncz, M. et al. (1987) Blood 69:219.
2. Scheuerer, B. et al. (2000) Blood 95:1158.
3. Perollet, C. et al. (1998) Blood 91:3289.
4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PF4