CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody

Recombinant Mouse CXCL3/DCIP‑1 (Catalog # 5568-CA) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL3/ DCIP‑1 (100 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse CXCL3/ DCIP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5568). The ND50 is typically 0.5-3 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse CXCL3/GRO gamma /CINC-2/DCIP-1
  Ala28-Ser100
  Accession # AAI17017
• Specificity: Detects mouse CXCL3/GRO gamma /CINC-2/DCIP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat CINC-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Cxcl3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody

• chemokine (C-X-C motif) ligand 3
• CINC-2
• CINC-2b
• C-X-C motif chemokine 3
• CXCL3
• Dcip1
• Gm1960
• GRO gamma
• GRO3 oncogene
• GRO3
• GROG
• GRO-gamma
• GRO-gamma(1-73)
• Growth-regulated protein gamma
• Macrophage inflammatory protein 2-beta
• melanoma growth stimulatory activity gamma
• MGSA gamma
• MIP2B
• MIP-2b
• MIP2-beta
• SCYB3

Background

CXCL3 is also known as MIP-2 beta (macrophage inflammatory protein 2 beta), or DCIP-1 (dendritic cell inflammatory protein-1) in mouse, CINC2 (cytokine-induced neutrophil attractant 2) in rat, and GRO-gamma (growth-regulated oncogene gamma) in humans (1, 2). It is an 8 kDa proinflammatory member of the CXC subfamily of heparin-binding chemokines, also called alpha chemokines (1‑4). The Glu-Leu-Arg (ELR) motif near the CXCL3 N-terminus confers angiogenic properties and distinguishes it from interferon-inducible ELR^- CXC chemokines, which are angiostatic (4). ELR⁺ and ELR^- chemokines use CXCR2 and CXCR3 receptors, respectively (3, 4). Mature mouse CXCL3 shares 88% and 57% amino acid (aa) sequence identity with rat and human CXCL3, respectively. Among mouse ELR⁺ chemokines, it shares 82% aa sequence identity with CXCL2/GRO-beta /MIP-2 and 34% - 58% with CXCL1/GRO-alpha /KC, CXCL5/ENA-78 and CXCL7/NAP-2. Due to their similar sequence and activity, CXCL2 and CXCL3 are sometimes referred to collectively as CXCL2/3, but are separate gene products (4‑6). Mouse CXCL3 expression is induced in macrophages and early in maturation of DC by bacterial products such as lipopolysaccharides, and other inflammatory mediators (1, 7). It is chemotactic for CXCR2‑expressing neutrophils, helping to recruit them to areas of inflammation (1, 7). ELR⁺ chemokines also elicit endothelial cell chemotaxis, stimulating angiogenesis and playing a role in tumor development (3, 4). ELR⁺ chemokines upregulated by ischemia play a role in ischemia-reperfusion injury (5, 6). A decoy receptor, DARC (Duffy antigen receptor for chemokines) competes with CXCR2 for ELR⁺ chemokine binding, thus downregulating their effect (8). Neutrophil influx may also be downregulated by MMP-12, which has been found to inactivate CXCL3 and other ELR⁺ chemokines by cleaving them at the ELR site (9). Over aa 28-100, mouse CXCL3 shares 87.8% aa identity with rat CINC2.

1. Nolan, K. F. et al. (2004) J. Immunol. 172:2201.
2. Modi, W. S. and T. Yoshimura (1999) Mol. Biol. Evol. 16:180.
3. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
4. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
5. Nesmelova, I. V. et al. (2008) J. Biol. Chem. 283:24155.
6. Maheshwari, A. et al. (2004) Fetal Pediatr. Pathol. 23:145.
7. Furuichi, K. et al. (2008) Front. Biosci. 13:4021.
8. Takano, K. and H. Nakagawa (2001) Inflamm. Res. 50:503.
9. Horton, L. W. et al. (2007) Cancer Res. 67:9791.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody (AF5568)(4)

Publications using AF5568

• Zhao, YF;Zuo, ZA;Li, ZY;Yuan, Y;Hong, SC;Fu, WG;Zhou, B;Wang, LX; Integrated multi-omics profiling reveals neutrophil extracellular traps potentiate Aortic dissection progression Nature communications 2024-12-30 [PMID: 39737994] (In vivo assay, Mouse)
• T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors Cancer Research, 2023-03-02;0(0):. 2023-03-02 [PMID: 36543251] (Neutralization, Mouse, Transgenic Mouse)
• N Zhang, Q Ma, Y You, X Xia, C Xie, Y Huang, Z Wang, F Ye, Z Yu, X Xie CXCR4-dependent macrophage-to-fibroblast signaling contributes to cardiac diastolic dysfunction in heart failure with preserved ejection fraction International journal of biological sciences, 2022-01-09;18(3):1271-1287. 2022-01-09 [PMID: 35173552] (Bioassay, Mouse)
• Wenting Liao, Michael J. Overman, Adam T. Boutin, Xiaoying Shang, Di Zhao, Prasenjit Dey et al. KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer Cancer Cell 4/1/2019 [PMID: 30905761]

Bioinformatics

• Gene Symbol: Cxcl3
• Uniprot:
  • AAI17017(Mouse)