CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody [Unconjugated]

Recombinant Rat CXCL3/CINC‑2 alpha (Catalog # 516‑CA) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/CINC‑2 alpha (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL3/CINC‑2 alpha / beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF516). The ND50 is typically 0.5-2.0 µg/mL.

• Reactivity: Rt
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
  Arg33-Ser100
  Accession # Q10746
• Specificity: Detects rat CXCL3/CINC-2 alpha / beta in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta , and rrCINC-3 is observed, less than 25% cross-reactivity with recombinant mouse (rm) KC, rmMIP-2, recombinant human (rh) GRO alpha , rhGRO beta , and rhGRO gamma is observed, and less than 10% cross-reactivity with rhIL-8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-2.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody [Unconjugated]

• chemokine (C-X-C motif) ligand 3
• CINC-2
• CINC-2b
• C-X-C motif chemokine 3
• CXCL3
• Dcip1
• Gm1960
• GRO gamma
• GRO3 oncogene
• GRO3
• GROG
• GRO-gamma
• GRO-gamma(1-73)
• Growth-regulated protein gamma
• Macrophage inflammatory protein 2-beta
• melanoma growth stimulatory activity gamma
• MGSA gamma
• MIP2B
• MIP-2b
• MIP2-beta
• SCYB3

Background

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody (AF516)(2)

Publications using AF516

• Piotrowska A, Rojewska E, Pawlik K et al. Pharmacological Blockade of Spinal CXCL3/CXCR2 Signaling by NVP CXCR2 20, a Selective CXCR2 Antagonist, Reduces Neuropathic Pain Following Peripheral Nerve Injury Front Immunol 9/26/2019 [PMID: 31616413]
• Miura TA, Wang J, Holmes KV, Mason RJ Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. Virology, 2007-09-05;369(2):288-98. 2007-09-05 [PMID: 17804032] (ICC, Rat)

Bioinformatics

• Gene Symbol: Cxcl3
• Uniprot:
  • Q10746()