Recombinant Virus CXCL2/GRO beta/MIP-2/CINC-3 Protein

• Reactivity: V-Vi
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Virus CXCL2/GRO beta/MIP-2/CINC-3 Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 70 amino acids corresponding to CXCL2/GRO beta/MIP-2/CINC-3 Source: E. coli

  Uniprot ID: Q98157

  Amino Acid Sequence: LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA

• Details of Functionality: CXCL2 Protein is fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL2/GRO beta/MIP-2/CINC-3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Virus CXCL2/GRO beta/MIP-2/CINC-3 Protein

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROb
• Gro-beta
• Growth-regulated protein beta
• Macrophage Inflammatory Protein 2
• Macrophage inflammatory protein 2-alpha
• Macrophage Inflammatory Protein
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

Viral MIP-2 is one of the three chemokine-like proteins expressed by the human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus, KSHV) and the other is ViMIP-1 and ViMIP-3. It shares 41 % and 48 % with human MIP-1alpha and ViMIP-1, respectively. Viral CXCL2 (MIP-2) has been shown to have antagonist activity towards a wide range of chemokine receptors and has functions of blocking infection by several different human immunodeficiency virus type 1 (HIV-1) strains. It may form part of the response to host defenses contributing to virus-induced neoplasia and may have relevance to KSHV and HIV-I interactions. Additionally, Viral MIP-2 has been shown to activate and chemoattract human eosinphils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL2
• Uniprot:
  • Q98157()