CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802)

Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/CINC‑3 (0.1 µg/mL) is neutral­ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC‑3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4‑2.0 µg/mL.

• Reactivity: Rt
• Applications: WB, B/N, ELISA(Cap)
• Clone: 123802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802) Summary

• Immunogen: E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3
  Ser32-Asn100
  Accession # P30348
• Specificity: Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant rat (rr) CXCL3 and less than 0.1% cross-reactivity with rrCXCL1, rrCINC-2 alpha , recombinant mouse (rm) CXCL9 or rmCXCL2 is observed. In Western blots, less than 3% cross-reactivity with recombinant human (rh) CXCL8, recombinant porcine CXCL8, or rrCINC-2 alpha and no cross‑reactivity with rrCXCL1, 3, rmCXCL1, 2, 6, 9, 10, 12, rhCXLC1, 2, 3, 5, 6, 7, 9,10, 11, 12 or 13 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.4-2.0 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Rat CXCL2/GRObeta /MIP-2/CINC-3 Biotinylated Antibody (Catalog number BAF525)
  Standard: Recombinant Rat CXCL2/GRObeta /MIP-2/CINC-3 (Catalog number 525-C3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802)

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROb
• Gro-beta
• Growth-regulated protein beta
• Macrophage inflammatory protein 2-alpha
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
2. Huang, S. et al. (1992) Am. J. Pathol. 141:981.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (MAB525)(4)

Publications using MAB525

• Piotrowska A, Rojewska E, Pawlik K et al. Pharmacological Blockade of Spinal CXCL3/CXCR2 Signaling by NVP CXCR2 20, a Selective CXCR2 Antagonist, Reduces Neuropathic Pain Following Peripheral Nerve Injury Front Immunol 9/26/2019 [PMID: 31616413]
• Michael S Runyon, Michael A Gellar, Nina Sanapareddy, Jeffrey A Kline, John A Watts Development and comparison of a minimally-invasive model of autologous clot pulmonary embolism in Sprague-Dawley and Copenhagen rats Thrombosis Journal 2010-12-01 [PMID: 20181228]
• Miura TA, Wang J, Holmes KV, Mason RJ Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. Virology, 2007-09-05;369(2):288-98. 2007-09-05 [PMID: 17804032] (ELISA Development, Rat)
• Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats. J. Mol. Cell. Cardiol., 2006-06-30;41(2):296-307. 2006-06-30 [PMID: 16814320] (ELISA Development, Rat)

Bioinformatics

• Gene Symbol: CXCL2
• Uniprot:
  • P30348()