| Reactivity | MuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids. |
| Standard Curve Range | 0.16 - 10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Specificity | This kit recognizes Mouse BRAK/CXCL14 in samples. No significant cross-reactivity or interference between Mouse BRAK/CXCL14 and analogues was observed. |
| Standard Curve Range | 0.16 - 10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | CV% < 5.57% |
| Intra-Assay | CV% < 4.9% |
| Spike Recovery | 93-112% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | CXCL14 |
| Reviewed Applications |
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| Storage | Storage is content dependent. |
| Components |
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reviewed by:
Anonymous |
ICC/IF | 07/19/2019 |
Summary
Comments
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reviewed by:
Anonymous |
07/19/2019 |
Summary
Comments
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Diseases for CXCL14/BRAK ELISA Kit (NBP2-70015)Discover more about diseases related to CXCL14/BRAK ELISA Kit (NBP2-70015).
| Pathways for CXCL14/BRAK ELISA Kit (NBP2-70015)View related products by pathway.
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PTMs for CXCL14/BRAK ELISA Kit (NBP2-70015)Learn more about PTMs related to CXCL14/BRAK ELISA Kit (NBP2-70015).
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| Anonymous 07/19/2019 |
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| Application: | ICC/IF | |
| Species: |
| Anonymous 07/19/2019 |
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| Gene Symbol | CXCL14 |
-img0001.jpg "Mouse CXCL14/BRAK ELISA Kit (Colorimetric) - Standard Curve Reference")
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Immunohistochemistry-Paraffin-NB600-1182-img0009.jpg)
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Western-Blot-NB600-1182-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Immunocytochemistry-Immunofluorescence-NB600-1182-img0016.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Western Blot: OXSM Antibody [NBP1-84730] - Analysis in control (vector only transfected HEK293T lysate) and OXSM over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/OXSM-Antibody-Western-Blot-NBP1-84730-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: OXSM Antibody [NBP1-84730] - Staining of human cell line A-431 shows localization to cytosol & mitochondria.](http://images.novusbio.com/images2/OXSM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84730-img0006.jpg)
![Immunohistochemistry-Paraffin: OXSM Antibody [NBP1-84730] - Staining of human cerebral cortex.](http://images.novusbio.com/images2/OXSM-Antibody-Immunohistochemistry-Paraffin-NBP1-84730-img0011.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Western-Blot-NBP1-92684-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: CXCL14/BRAK Antibody [NBP1-31398] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/CXCL14-BRAK-Antibody-Western-Blot-NBP1-31398-img0011.jpg "Western Blot: CXCL14/BRAK Antibody [NBP1-31398] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE; antibody diluted at 1:1000.")