CXCL13/BLC/BCA-1 Antibody (53610) [Allophycocyanin]

Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were stained with Mouse Anti-Human CXCL13/BLC/BCA-1 APC-conjugated Monoclonal Antibody (Catalog # IC801A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

CXCL13/BLC/BCA-1 Antibody (53610) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human CXCL13/BLC/BCA-1
  Val23-Arg94
  Accession # O43927
• Specificity: Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL-8, rhCXCL10, rhCXCL9, rhCXCL12/SDF-1 alpha , or rhCXCL12/SDF-1 beta .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL13
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody (53610) [Allophycocyanin]

• ANGIE
• ANGIE2
• B cell-attracting chemokine 1
• B lymphocyte chemoattractant
• BCA1
• BCA-1
• BCA1B-cell chemoattractant
• BCA-1CXC chemokine BLC
• B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
• BLC
• BLCSmall-inducible cytokine B13
• BLR1L
• B-lymphocyte chemoattractant
• chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
• chemokine (C-X-C motif) ligand 13
• C-X-C motif chemokine 13
• CXCL13
• SCYB13
• SCYB13B-cell-attracting chemokine 1
• small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

1. Gunn, M.D. et al. (1998) Nature, 391:799.
2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
3. Forster, R. et al. (1996) Cell 87:1037.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL13/BLC/BCA-1 Antibody (IC801A)(5)

Publications using IC801A

• Z Xin, M Lin, Z Hao, D Chen, Y Chen, X Chen, X Xu, J Li, D Wu, Y Chai, P Wu The immune landscape of human thymic epithelial tumors Nature Communications, 2022-09-17;13(1):5463. 2022-09-17 [PMID: 36115836] (Flow Cytometry, Human)
• M Ukita, J Hamanishi, H Yoshitomi, K Yamanoi, S Takamatsu, A Ueda, H Suzuki, Y Hosoe, Y Furutake, M Taki, K Abiko, K Yamaguchi, H Nakai, T Baba, N Matsumura, A Yoshizawa, H Ueno, M Mandai CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer JCI Insight, 2022-06-22;0(0):. 2022-06-22 [PMID: 35552285] (Flow Cytometry, Human)
• H Yoshitomi, S Kobayashi, A Miyagawa-H, A Okahata, K Doi, K Nishitani, K Murata, H Ito, T Tsuruyama, H Haga, S Matsuda, J Toguchida Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments Nat Commun, 2018-09-19;9(1):3762. 2018-09-19 [PMID: 30232328] (Flow Cytometry, Human)
• Kobayashi S, Watanabe T, Suzuki R, Furu M, Ito H, Ito J, Matsuda S, Yoshitomi H TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur J Immunol, 2015-11-24;46(2):360-71. 2015-11-24 [PMID: 26541894] (Flow Cytometry, Human)
• Cohen K, Dugast A, Alter G, McElrath M, Stamatatos L HIV-1 single-stranded RNA induces CXCL13 secretion in human monocytes via TLR7 activation and plasmacytoid dendritic cell-derived type I IFN. J Immunol, 2015-02-09;194(6):2769-75. 2015-02-09 [PMID: 25667414] (ICC, Human)

Reviews for CXCL13/BLC/BCA-1 Antibody (IC801A) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 23778140
• Species: Human

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 24022618
• Species: Human

Bioinformatics

• Gene Symbol: CXCL13
• Uniprot:
  • O43927()