CXCL11/I-TAC Antibody [Unconjugated]

Recombinant MouseCXCL11/I‑TAC (Catalog #572-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/I‑TAC (200 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL11/I-TAC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF572). The ND50 is typically 2-12 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL11/I-TAC Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL11/I-TAC
  Phe22-Met100
  Accession # Q9JHH5
• Specificity: Detects mouse CXCL11/I-TAC in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human CXCL11, recombinant mouse (rm) BLC, rmCRG‑2, rmGCP‑2, rmMIG, rmPF4, and rmSDF‑1 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL11
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 5-25 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CXCL11/I-TAC Antibody (Catalog # AF572)
  ELISA Detection: Mouse CXCL11/I-TAC Biotinylated Antibody (Catalog # BAF572)
  Standard: Recombinant Mouse CXCL11/I-TAC (Catalog # 572-MC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL11/I-TAC Antibody [Unconjugated]

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

CXCL11 (also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1) is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (CRG-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine, and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL11/I-TAC Antibody (AF572)(5)

Publications using AF572

• L Pouzol, A Sassi, N Baumlin, M Tunis, DS Strasser, F Lehembre, MM Martinic CXCR7 Antagonism Reduces Acute Lung Injury Pathogenesis Oncogene, 2021-11-05;12(0):748740. 2021-11-05 [PMID: 34803691] (ELISA Detection, Mouse)
• Malte Puchert, Christian Koch, Konstanze Zieger, Jürgen Engele Identification of CXCL11 as part of chemokine network controlling skeletal muscle development Cell and Tissue Research 5/1/2021 [PMID: 33502606]
• T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency Cell Stem Cell, 2019-04-25;0(0):. 2019-04-25 [PMID: 31031137] (ICC, Mouse)
• Schenkel J, Fraser K, Vezys V, Masopust D Sensing and alarm function of resident memory CD8(+) T cells. Nat Immunol, 2013-03-31;14(5):509-13. 2013-03-31 [PMID: 23542740] (IHC, Mouse)
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCL11
• Uniprot:
  • Q9JHH5()