CXCL11/I-TAC Antibody (87328) [Unconjugated]

Recombinant Human CXCL11/I‑TAC (672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND50 is typically 0.0400‑0.480 µg/mL.

CXCL10 cleavage by GP63 occurs between positions A81 and I82.(B) GP63 selectively cleaves chemokine ligands of the CXCR3 receptor. Conditioned media from L. major WT, delta gp63, and delta gp63+1 was incubated with human recombinant chemokines for 12 h and product detected by western blot. Cleavage is only detected for the CXCL9/10/11 family. Representative blots are shown from at least two independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31440475), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 87328
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL11/I-TAC Antibody (87328) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL11/I-TAC
  Phe22-Phe94
  Accession # O14625
• Specificity: Detects human CXCL11/I-TAC in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL5, 7, 8, 9, or 10. In Western blots, this antibody shows less than 1% cross-reactivity with rhCXCL1, 2, 3, 5, 6, 7, IL-8/CXCL8, 9, 10, 13, CXCL12/SDF-1 alpha , CXCL12/SDF-1 beta , rmCXCL1, 2, 6, 9, 10, CXCL12/SDF-1 alpha , rmCXCL13, rrCXCL1, CXCL3/CINC-2 alpha , CXCL3/CINC-2 beta , or rpIL-8/CXCL8.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL11
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.0400-0.480 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CXCL11/I-TAC Biotinylated Antibody (Catalog number BAF672) Standard: Recombinant Human CXCL11/I-TAC (Catalog number 672-IT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL11/I-TAC Antibody (87328) [Unconjugated]

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

1. Cole, K. et al. (1998) J. Exp. Med. 187:2009.
2. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
3. Lou, Y. et al. (1998) J. Neurovirol. 4:575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL11/I-TAC Antibody (MAB672)(14)

Publications using MAB672

• Wenshuo Zhang, Pang Lyu, Darja Andreev, Yewei Jia, Fulin Zhang, Aline Bozec Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma Frontiers in Cell and Developmental Biology 12/12/2022 [PMID: 36578780]
• Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer Cancer Immunology Research 7/1/2022 [PMID: 35561311]
• Alejandro L. Antonia, Kyle D. Gibbs, Esme D. Trahair, Kelly J. Pittman, Amelia T. Martin, Benjamin H. Schott et al. Pathogen Evasion of Chemokine Response Through Suppression of CXCL10 Frontiers in Cellular and Infection Microbiology 8/7/2019 [PMID: 31440475]
• X Yuan, Y Liu, G Lee, Z Lan, M Ma, H Lee, J Kong, J Sun, G Hou, X Hou, Y Ma, F Ren, F Zhou, S Gao Blockade of Immune Checkpoint B7-H4 and Lysine Demethylase 5B in Esophageal Squamous Cell Carcinoma Confers Protective Immunity against P. gingivalis Infection Cancer Immunol Res, 2019-07-26;0(0):. 2019-07-26 [PMID: 31350278] (Bioassay, Human)
• Mudan Zhang, Xu Deng, Xinmeng Guan, Lanlan Geng, Ming Fu, Binman Zhang et al. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4 Frontiers in Immunology 12/19/2018 [PMID: 30619292]
• Daniel P. Harris, Unnikrishnan M. Chandrasekharan, Smarajit Bandyopadhyay, Belinda Willard, Paul E. DiCorleto PRMT5-Mediated Methylation of NF-kappa B p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-alpha and IFN-gamma Costimulation PLOS ONE 2/22/2016 [PMID: 26901772]
• Daniel P. Harris, Unnikrishnan M. Chandrasekharan, Smarajit Bandyopadhyay, Belinda Willard, Paul E. DiCorleto PRMT5-Mediated Methylation of NF-kappa B p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-alpha and IFN-gamma Costimulation PLOS ONE 2016-02-22 [PMID: 26901772] (Western Blot, Human)
• Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014-02-22;14(0):118. 2014-02-22 [PMID: 24559071] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Neutralization, Human)
• Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Invest. Ophthalmol. Vis. Sci., 2009-10-22;51(2):643-50. 2009-10-22 [PMID: 19850844] (IHC-P, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007-09-15;179(6):4272-82. 2007-09-15 [PMID: 17785868] (ICC, Immunodepletion, Human)
• Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow. Am. J. Pathol., 2005-09-01;167(3):887-99. 2005-09-01 [PMID: 16127166] (IHC, Western Blot, Human)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (IHC, Human)
• Liu L, Jarjour NN, Busse WW, Kelly EA Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24. 2004-03-04 [PMID: 15001464] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CXCL11
• Uniprot:
  • O14625()