CXCL11/I-TAC Antibody (131327)

Recombinant Mouse CXCL11/I‑TAC (Catalog # 572-MC) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/I‑TAC (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB572). The ND50 is typically 0.4‑2.0 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 131327
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CXCL11/I-TAC Antibody (131327) Summary

• Immunogen: E. coli-derived recombinant mouse CXCL11/I-TAC
  Phe22-Met100
  Accession # Q9JHH5
• Specificity: Detects mouse CXCL11/I-TAC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CXC1, 2, 6, 9, 10, CXCL12/SDF-1 alpha , 13, 14, recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, CXCL12/SDF-1 alpha , CXCL12/SDF-1 beta , 13, 14, 15, recombinant rat CXCL1, 2, CXCL3/CINC2 alpha , CXCL3/CINC-2 beta , 5, or recombinant porcine CXCL8 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCL11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.4-2.0 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL11/I-TAC Antibody (131327)

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

CXCL11 (also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1) is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) precursor protein with a putative 21 aa signal peptide that is cleaved to yield a 79 aa mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (CRG-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine, and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL11/I-TAC Antibody (MAB572)(4)

Publications using MAB572

• L Pouzol, A Sassi, N Baumlin, M Tunis, DS Strasser, F Lehembre, MM Martinic CXCR7 Antagonism Reduces Acute Lung Injury Pathogenesis Oncogene, 2021-11-05;12(0):748740. 2021-11-05 [PMID: 34803691] (ELISA Capture, Mouse)
• Del Debbio C, Santos M, Yan C, Ahmad I, Hamassaki D Rho GTPases control ciliary epithelium cells proliferation and progenitor profile induction in vivo. Invest Ophthalmol Vis Sci, 2014-04-21;55(4):2631-41. 2014-04-21 [PMID: 24692128] (IHC, Mouse)
• Moore K, Bryant ZJ, Ghatnekar G et al. A synthetic connexin 43 mimetic peptide augments corneal wound healing. Exp Eye Res. [PMID: 23876491]
  
  Details:
  Citation using the Unpurified form of this antibody.
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CXCL11
• Uniprot:
  • Q9JHH5()