Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CXCL10/IP-10/CRG-2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 77 amino acids corresponding to CXCL10/IP-10/CRG-2 Source: E. coli

  Uniprot ID: P17515

  Amino Acid Sequence: IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP

• Details of Functionality: CXCL10/IP-10/CRG-2 protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1- 10.0 ng/ml in the presence of IL-2.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL10
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL10/IP-10/CRG-2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• IP-10/CXCL10
• mob-1
• SCYB10

Background

CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene, and in murine it is also named the CRG-2 gene. The gene was originally identified as an immediate early gene induced in response to macrophage activation. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. Murine CXCL10 shares approximately 67 % amino acid sequence identity with human CXCL10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL10
• Uniprot:
  • P17515()
  • P17515()
  • P17515()